Exosome Preparation from Urine

  1. Collect urine in sterile 50-ml plastic centrifuge tubes containing 4.2 ml of a proteinase inhibitor mixture
  2. Mix and centrifuge at 17,000 x g for 15 min at 4°C to remove whole cells, large membrane fragments, and other debris
  3. Centrifuge at 200,000 x g for 1 h at 4°C to obtain a low-density membrane pellet (exosome)
reference : Trairak Pisitkun, Rong-Fong Shen, and Mark A. Knepper. Identification and proteomic profiling of exosomes in human urine. PNAS 101:13368-13373, 2004