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Human Kidney & Urine Proteome Project
"Towards Standards for Urine Proteomics"

Saturday, Oct 6, 14:00-16:00, Jangbo Hall 333, COEX, Seoul, Korea
   
14:00-14:05 Introduction
Tadashi Yamamoto, Chair of HKUPP, Niigata University
14:05-14:20 Urinary proteomics of high-molecular-mass proteins by 2-DE with agarose gels in the first dimension (Agarose 2-DE) and LC-MS/MS
Hiroshi Okusa1, Yoshio Kodera2 ,3, Ryojiro Kondo2, Kazumasa Matsumoto1, Masatsugu Iwamura1 Masamichi Oh-Ishi2, Shiro Baba1, and Tadakazu Maeda2 ,3
1 Department of Urology, Kitasato University School of Medicine, 2 Department of Physics, Kitasato University School of Science, and 3Center for Disease Proteomics, Kitasato University School of Science,Sagamihara, Japan
14:20-14:35 The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins
Jun Adachi1 ,2, Nagaraj, Nagarjuna1
1Department of Proteomics and Signal Transduction, Max-Planck Institute for Biochemistry, Germany, and 2Current address: Graduate School of Global Environmental Studies, Kyoto University, Japan
14:35-14:50 An attempt to understand kidney functions by comparing serum and urine proteomes
Lulu Jia, Ling Zhang, Chen Shao, Eli Song, Wei Sun and Youhe Gao
Department of Pathophysiology, Proteomics Research Center, Institute of Basic Medical Sciences, Peking Union Medical College, Beijing, China
14:50-15:05 Identification of a processed ubiquitin form as differentially excreted protein in diabetic nephropathy patients by urinary proteomics
Hassan Dihazi, and Gerhard A. Muller
Nephrology & Rheumatology, Georg-August University Goettingen, Goettingen, Germany
15:05-15:20 Two-dimensional gel electrophoresis-based proteome analysis of normal human urine proteome: optimization of urine sample handling and quantitative difference between healthy children and adults
Yutaka Yoshida1, Yoshimi Nagasaka1, Izumi Taguchi1, Masahito Miyamoto1, Bo Xu1, Ying Zhang1, Kenji Miyazaki2, Akira Tsugita2, Eishin Yaoita1, Hidehiko Fujinaka1, 3, Tadashi Yamamoto1
1Department of Structural Pathology, Institute of Nephrology, Niigata University, Niigata, 2Proteomics Research Center, Fundamental and Environmental Res. Labs. NEC Corp., Tsukuba, and 3Institute for Clinical Research, Niigata National Hospital, Kashiwazaki, Japan.
15:20-15:45 Issues of concern in urinary proteomics and initial recommendations
Visith Thongboonkerd
Medical Molecular Biology Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Maida University, Bangkok, Thailand.
15:45-16:00 Discussion