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Agilent Technologies| 2nd Workshop of Human Kidney & Urine Proteome Project (HKUPP) "Towards Standards for Urine Proteomics" |
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| 15:00-17:00 | Session 1 Towards standards for urine proteomics |
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| Co-chairs:Robyn G Langham (University of Melbourne, Australia) and Visith Thongboonkerd (Mahidol University, Thailand) | |||||
| 15:00-15:10 | Introduction | ||||
| Tadashi Yamamoto Chair of HKUPP, Niigata University, Niigata, Japan |
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| 15:10-15:25 | Urinary profiling by MALDI MS and 2DE-based approaches: Optimizing urine processing and protein sample preparation | ||||
| P Zerefos, M. Makridakis, and A. Vlahou Biomedical Research Foundation of the Academy of Athens, Greece |
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| 15:25-15:40 | Standardization of peptidome profiling of human urine by SELDI-TOF MS and magnetic bead separation MS | ||||
| Hassan Dihazi, Gerhard A Mueller Clinical Proteomics Laboratories, Nephrology and Rheumatology, Georg-August University Goettingen, Goettingen, Germany |
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| 15:40-15:55 | CE-MS analysis of low molecular weight urinary proteins | ||||
| JP Schanstra1, and Herald Mischak2 1Inserm U858, 12MR, Renal and Cardiac Remodeling, Toulouse, France, and 2Mosaiques Diagnostics and Therapeutics, Hanover, Germany |
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| 15:55-16:10 | Some topics of 2-DE based proteomics of urinary proteins: 2D DIGE analysis of urinary proteins of healthy children and adults and agarose 2-DE analysis of HMW proteins (tentative) | ||||
| Yutaka Yoshida1, Yoshimi Nagasaka1, Yoshio Kodera2, Masamichi Oh-ishi2, Tadakazu Maeda2 and Tadashi Yamamoto1 1Department of Structural Pathology, Institute of Nephrology, Niigata University, Niigata, Japan and 2Department of Physics, Kitasato University School of Science, Sagamihara, Japan. |
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| 16:10-16:40 | Issues of concern in urinary proteomics and initial recommendation: a draft for standard protocols of urine proteomics (tentative) | ||||
| Visith Thongboonkerd Medical Molecular Biology Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. |
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| 16:40-17:10 | Free discussion | ||||
| 17:10-17:20 | Break | ||||
| 17:20-18:00 | Session 2 Discussion: action plan of HKUPP |
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| Co-chairs: Tadashi Yamamoto (Niigata University, Japan) and Pierre Ronco (INSERM 702, Paris) | |||||
| To presenters | |||||
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