HKUPP

Kidney and Urine Proteomics

Jon B Klein (University of Louisville, Louisville, SA, USA)

Kidney and Urine Proteome

Chair: Mark A Knepper (NHLBI, NIH, Bethesda, MD, USA)

Robyn G Langham (University of Melbourne, Melbourne, Australia)

Pierre Ronco (INSERM 702, Paris, France)

19:35-19:45 (10min)

Quantitative Phosphoproteomics of IMCD Cells: Regulation of Aquaporin-2 Phosphorylation at Two Sites

Jason Hoffert (NHLBI, NIH, Bethesda, USA)

19:45-19:55 (10min)

Profiling of Glomerulus Proteome of Normal Human Kidney: Proteomic Approach by 2D Protein Prefractionation and LC-MS/MS

Yutaka Yoshida (Niigata University, Niigata, Japan)

Masahito Miyamoto (Niigata University, Niigata, Japan)

Tadashi Yamamoto (Niigata University, Niigata, Japan)

Coffee Break (10 min)

20:05-20:15 (10min)

Galectin 1, A Biomarker for Renal Cell Cancer

Gerhard A Muller (University of Gottingen, Gottingen, Germany)

20:15-20:25 (10min)

High Resolution Urinary Proteome Analysis by Capillary-Electrophoresis Coupled Mass Spectrometry for Clinical/Diagnostic Application

Harald Mischak (Mosaiques Diagnostics and Therapeutics AG, Hanover, Germany)

20:25-21:35 (10min)

Urinary Exosomes: Promise and Practicality

Trairak Pisitkun (NHLBI, NIH, Bethesda, MD, USA)

Patricia Gonzales (NHLBI, NIH, Bethesda, MD, USA)

Rong-Fong Shen (NHLBI, NIH, Bethesda, MD, USA)

Mark A. Knepper (NHLBI, NIH, Bethesda, MD, USA)

20:35-20:45 (10min)

Systematic Evaluation of Sample Preparation Methods for Gel-Based Human Urinary Proteomics

Visith Thongboonkerd
(FRCPT Siriraj Hospital, Mahidol University, Bangkok, Thailand)

20:45-20:55 (10min)

MI2DG: A Web-Based Resource to Share Details of Sample Preparation for Analysis of Urine and Kidney Proteomes by 2DE

John M Arthur

Michael G. Janech

Jonas S. Almeida
(Medical University of South Carolina, Charleston, SC and University of Texas MD Anderson Cancer Center, Houston, TX, USA)

20:55-21:05 (10min)

Characterization and Standardization of the Urinary Proteome by Two Complementary Proteomic Approaches : 2-D PAGE Combined to MS and SELDI-TOF-MS.
Loreto Gesualdo¹, Massimo Papale¹, Marta Centra¹, Maria Teresa Rocchetti¹, Grazia Bortone¹, Clelia Prattichizzo¹, Elena Ranieri¹, Carmen Palermo², Diego Centonze², Bradley J. Thatcher³, and Salvatore Di Paolo⁴

Molecular Medicine Center, Section of Nephrology, Department of Biomedical Sciences and Bioagromed, Faculty of Medicine, University of Foggia, Foggia

Department of Analitical Sciences and Bioagromed, Faculty of Agricultural Sciences, University of Foggia, Foggia

Ciphergen Biosystems , ProteinChip Demo Lab, Naples

Department of Emergency and Organ Transplants, Section of Nephrology, University of Bari, Italy.

21:05-21:15 (10min)

Urinary Profiling by MALDI MS and Preparative 2DE-Based Approaches: Optimizing Protein Sample Preparation

P. Zerefos (Foundation for Biomedical Research of the Academy of Athens, Greece)

J. Prados (University of Geneva, Switzerland)

A. Kalousis (University of Geneva, Switzerland)

K. Stravodimos (Urologic Clinic, Laikon Hospital, Medical School of Athens, Greece)

M. Fountoulakis (Hoffmann- La Roche, Switzerland)

A. Vlahou (Foundation for Biomedical Research of the Academy of Athens, Greece)

Free Discussion (15min)

21:15-21:30

*******************************************************************

To Presenters

Only LCD computer projector is used for all presentations. We would like to ask you to bring your own laptop computer or bring a USB memory stick in which your presentation file is saved. For the workshop, laptop computers will be prepared which runs Windows XP and PowerPoint 2003.
It would be greatly appreciated if you could agree to save your presentation file in our computers for further discussion after the workshop.